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wild type d3 es cell line  (ATCC)


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    ATCC wild type d3 es cell line
    Wild Type D3 Es Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+d3+es+cell+line/TIBx%3B+Epithelial+liver%3B+Mouse/pmc03639543-42-1-6
    Average 97 stars, based on 2806 article reviews
    wild type d3 es cell line - by Bioz Stars, 2026-09
    97/100 stars

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    Cell Culture:

    Article Title: Mineralocorticoid receptor overexpression facilitates differentiation and promotes survival of embryonic stem cell-derived neurons
    Article Snippet: .. The wild-type D3 ES cell line (ATCC no. CRL-11693) and the hMR ES cells were grown on 0.1% gelatin-coated plates (Sigma-Aldrich, Lyon, France) and on feeder cells (STO Neomycin LIF, kindly provided by Dr Alan Bradley, The Wellcome Trust Sanger Institute, UK) pretreated with 15 μg/ml mitomycin C (Sigma-Aldrich) for 4 h. Cells were cultured at 37°C in a humidified incubator in presence of 5% CO 2 . .. Reagents ES medium was composed of DMEM (PAA, Les Mureaux, France) containing 15% fetal calf serum (FCS specifically tested for ES culture (AbCys SA, Paris, France), 1X non-essential amino acids (PAA), 2 mM glutamine (PAA), 100 U/ml penicillin (PAA), 100 μg/ml streptomycin (PAA), 20 mM HEPES (PAA) and 100 μM β-mercaptoethanol (Sigma-Aldrich).



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